Section on Protein Biogenesis

Selected Publications

Note: * indicates shared first authorship and # indicates shared corresponding authorship.

1990's - 2001 | 2002 | 2003 | 2004 | 2005 | 2006 | 2007 | 2008 | 2009

2009

Ashok, A. and Hegde, R.S. (2009) Selective Processing and Metabolism of Disease-Causing Mutant Prion Proteins. PLoS Pathogens, 5:e1000479. PDF

Chakrabarti, O. and Hegde, R.S. (2009) Functional depletion of Mahogunin by cytosolically exposed prion protein contributes to neurodegeneration. Cell, 137:1136-1147. PDF

Chakrabarti, O., Ashok, A., and Hegde, R.S. (2009) Prion protein biosynthesis and its emerging role in neurodegeneration. Trends Biochem. Sci., 34:287-95. PDF

Polgar, O., Ediriwickrema, L.S., Robey, R.W., Sharma, A., Hegde, R.S., Li, Y., Xia, D., Ward, Y., Dean, M., Ozvegy-Laczka, C., Sarkadi, B., and Bates, S.E. (2009) Arginine 383 is a crucial residue in ABCG2 biogenesis. Biochim. Biophy. Acta, 1788(7):1434-43. PDF

Hegde, R.S. (2009)
Protein maturation and processing at the endoplasmic reticulum. In: The Liver: Biology and Pathobiology. 5th Edition. Edited by I. A. Arias et al., Wiley-Blackwell, In press.
Sharma, A.S., Mariappan, M., Appathurai, S., and Hegde, R.S. (2009)
In vitro dissection of protein translocation into the mammalian endoplasmic reticulum. Methods Mol. Biol. In press.

2008

Kang, S.W. and Hegde, R.S. (2008)
Lighting up the stressed ER. Cell, 135(5):787-9.
PDF
Rane, N.S., Kang, S.W., Chakrabarti, O., Feigenbaum, L., and Hegde, R.S. (2008)
Chronic prion protein degradation via pre-emptive quality control causes neurodegeneration. Dev. Cell, 15:359-370.
PDF
Hegde, R.S. and Kang, S.W. (2008)
The concept of translocational regulation. J. Cell Biol. 182:225-232.
PDF
Menetret, J.F., Hegde, R.S., Aguiar, M., Gygi, S.P., Park, E., Rapoport, T.A., and Akey, C.W. (2008)
Single copies of Sec61 and TRAP associate with a non-translating mammalian ribosome. Structure, 16:1126-1137. PDF
Ashok, A. and Hegde, R.S. (2008)
Retrotranslocdation of prion proteins from the ER by inhibition of GPI signal sequence transamidation. Mol. Biol. Cell. 19:3463-3476.
PDF
Ashok, A. and Hegde, R.S. (2008)
Cytotoxicity of aberrant proteins. In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: DOI: 10.1002/9780470015902.a0020887. PDF

2007

MacKinnon, A.L., Garrison, J.L., Hegde, R.S., and Taunton J. (2007)
Photo-leucine incorporation reveals the target of a cyclodepsipeptide inhibitor of cotranslational translocation. J. Am. Chem. Soc. 129:14560-1. PDF
*Rutkowski, D.T., *Kang, S.W., Goodman, A.G., Garrison, J.L., Taunton, J., Katze, M.G., Kaufman, R.J., and Hegde, R.S. (2007)
The Role of p58IPK in Protecting the Stressed Endoplasmic Reticulum. Mol. Biol. Cell, 18:3681-91. PDF
Ong, H. L., Liu, X., Tsaneva-Atanasova, K., Singh, B. B., Bandyopadhyay, B. C., Swaim, W. D., Russell, J. T., Hegde, R. S., Sherman, A., and Ambudkar, I. S. (2007)
Relocalization of STIM1 for activation of store-operated Ca2+ entry is determined by the depletion of subplasma membrane endoplasmic reticulum Ca2+ store. J. Biol. Chem., 282:12176-85. PDF
Stefanovic, S. and Hegde, R. S. (2007)
Identification of a targeting factor for post-translational membrane protein insertion into the ER. Cell, 128:1147-1159. PDF
Alken, M. and Hegde, R. S. (2007)
The translocation apparatus of the endoplasmic reticulum. In: Molecular machines involved in protein transport across cellular membranes, ed. R. Dalbey, C. Koehler, and F. Tamanoi. Academic press/Elsevier. PDF
Ong, H. L., Liu, X., Sharma, A., Hegde, R. S., and Ambudkar, I. (2007)
Intracellular Ca2+ release via the ER translocon activates store-operated calcium entry. Pflugers Arch. 453:797-808. PDF

2006

*Kang, S. W., *Rane, N. S., Kim, S. J., Garrison, J. L., Taunton, J., and Hegde, R. S. (2006)
Substrate-specific translocational attenuation during ER stress defines a pre-emptive quality control pathway. Cell, 127:999-1013.PDF
Hegde, R. S. and Bernstein, H. D. (2006)
The surprising complexity of signal sequences. Trends Biochem. Sci. 31:563-71. PDF
Ashok, A. and Hegde, R. S. (2006)
Prions and retroviruses: an endosomal rendezvous? EMBO Rep. 7:685-687. PDF
Snapp, E. L. and Hegde, R. S. (2006)
Rational design and evaluation of FRET experiments to measure protein proximities in cells. In Curr. Prot. in Cell Biol., ed. J. S. Bonifacino et al., Wiley PDF
Snapp, E. L., Sharma, A., Lippincott-Schwartz, J., and Hegde, R. S. (2006)
Monitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells. Proc. Natl. Acad. Sci., 103(17):6536-6541. PDF
Chakrabarti, O. and Hegde, R. S. (2006)
Trafficking of the cellular Prion protein and its role in neurodegeneration. In: Protein trafficking in the neuron, ed. A. Bean, Elsevier, 413-436. PDF

2005

*Shaffer, K. L., *Sharma, A., Snapp, E. L., and Hegde, R. S. (2005)
Regulation of protein compartmentalization expands the diversity of protein function. Dev. Cell, 9:545-554.PDF
Brambillasca, S., Yabal, M., Soffientini, P., Stefanovic, S., Makarow, M., #Hegde, R. S., and #Borgese, N. (2005)
Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition. EMBO J., 24:2533-2542. PDF
Garrison, J. L., Kunkel, E. J., #Hegde, R. S., and #Taunton, J. (2005)
A substrate-specific inhibitor of protein translocation into the endoplasmic reticulum. Nature, 436:285-289. PDF
*Menetret, J. F., *Hegde, R. S., Heinrich, S. U., Chandramouli, P., Ludtke, S. J., Rapoport, T. A., and Akey, C. W. (2005)
Architecture of the ribosome-channel complex derived from native membranes. J. Mol. Biol., 348:445-457. PDF
Hegde, R. S. (2005)
Protein translocation across the endoplasmic reticulum. In: Protein movement across membranes, pp. 1-18. ed. J. Eichler, Landes Bioscience, Georgetown, TX. PDF
Hegde, R. S. and Rane, N. S. (2005)
The molecular basis of prion protein-mediated neuronal damage. In: Neurodegeneration and Prion Disease, pp. 407-450, ed. D. R. Brown, Springer, NY, NY. PDF
Levine, C. G., Mitra, D., Sharma, A., Smith, C. L., and Hegde, R. S. (2005)
The efficiency of protein compartmentalization into the secretory pathway. Mol. Biol. Cell, 16:279-291. PDF

2004

Rane, N., Yonkovich, J., and Hegde, R. S. (2004)
Protection from cytosolic prion protein toxicity by modulation of protein translocation. EMBO J., 23:4550-4559. PDF
*Karsten, V., *Hegde, R. S., Sinai, A. P., Yang, M., and Joiner, K. (2004)
Transmembrane domain modulates sorting of membrane proteins in toxoplasma gondii. J. Biol. Chem. 279:26052-26057. PDF
Snapp, E. L., Reinhart, G. A., Bogert, B. A., Lippincott-Schwartz, J., and Hegde, R. S. (2004)
The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells. J. Cell Biol. 164:997-1007. PDF
Tremblay P., Ball, H. L., Kaneko, K., Groth, D., Hegde, R. S., Cohen, F. E., DeArmond, S. J., Prusiner, S. B., and Safar, J. G. (2004)
Mutant PrPSc conformers induced by a synthetic peptide and various prion strains. J. Virol., 78:2088-2099. PDF

2003

Snapp, E. L., Hegde, R. S., Francolini, M., Lombardo, F., Colombo, S., Pedrazzini, E., Borgese, N., and Lippincott-Schwartz, J. (2003)
Formation of stacked ER cisternae by low affinity protein interactions. J. Cell Biol.,163:257-69. PDF
Hegde, R. S. and Rane, N. S. (2003)
Prion protein trafficking and the development of neurodegeneration. Trends Neurosci., 26:337-339. PDF
Fons, R. D., Bogert, B. A., and Hegde, R. S. (2003)
Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane. J. Cell Biol. 160:529-39. PDF

2002

Hegde, R. S. (2002)
Targeting and beyond: new roles for old signal sequences. Mol. Cell, 10:697-98. PDF
Kim, S. J. and Hegde, R. S. (2002)
Cotranslational Partitioning of Nascent Prion Protein into Multiple Populations at the Translocation Channel. Mol. Biol. Cell, 13:3775-3786. PDF
Kim, S. J., Mitra, D., Salerno, J. S., and Hegde, R. S. (2002)
Signal sequences control gating of the protein translocation channel in a substrate-specific manner. Dev. Cell,2:207-217. PDF

2001 - 1996

Kim, S. J., Rahbar, R., and Hegde, R. S. (2001)
Combinatorial control of prion protein biogenesis by the signal sequence and transmembrane domain. J. Biol. Chem. 276:26132-26140. PDF
Rutkowski, D. T., Lingappa, V. R. and Hegde, R. S. (2001)
Substrate-specific regulation of the ribosome-translocon junction by N-terminal signal sequences. Proc. Natl. Acad. Sci. 98:7823-7828. PDF
Hegde, R. S., Tremblay, P., Groth, D., DeArmond, S. J., Prusiner, S. B. and Lingappa, V.R. (1999)
Transmissible and genetic prion diseases share a common pathway of neurodegeneration. Nature, 402:822-826. PDF
Hegde, R. S. and Lingappa, V. R. (1999)
Regulation of protein biogenesis at the endoplasmic reticulum membrane. Trends Cell Biol., 9:132-137. PDF
Hegde, R. S., Voigt, S., and Lingappa, V. R. (1998)
Regulation of protein topology by trans-acting factors at the endoplasmic reticulum. Mol. Cell, 2:85-91. PDF
Hegde, R. S., Voigt, S., Rapoport, T. A., and Lingappa, V. R. (1998)
TRAM regulates the exposure of nascent secretory proteins to the cytosol during translocation into the endoplasmic reticulum. Cell, 92:621-631. PDF
Hegde, R. S., Mastrianni, J. A., Scott, M. R., DeFea, K. A., Tremblay, P., Torchia, M., DeArmond, S. J., Prusiner, S. B., and Lingappa, V. R. (1998)
A transmembrane form of the prion protein in neurodegenerative disease. Science, 279:827-834. PDF
Hegde, R. S. and Lingappa, V. R. (1997)
Membrane protein biogenesis: regulated complexity at the endoplasmic reticulum. Cell, 91: 575-582. PDF
Hegde, R. S. and Lingappa, V. R. (1996)
Sequence-specific alteration of the ribosome-membrane junction exposes nascent secretory proteins to the cytosol. Cell, 85:217-228. PDF